A Complete Guide on Microbiological Dipslide

Dipslide

A dipslide is a test carried out by a microbial testing center to know the presence of microorganisms in liquids. Testing the microbial activity in liquid-based systems has, over time, become one of the most critical tests. Luckily, one can carry out the test independently as a variety of dipslides are readily available in the market at very affordable prices.

Fungi and Bacteria test kit

Most bacteria and fungi test kit suppliers avail it with everything that you need to begin testing. It is opposed to purchasing items separately, as you often forget some of the items.

About the bacteria and fungi dipslide kit

This dipslide usually test for anaerobic bacteria and fungi. With the red spot dye, it is straightforward to interpret the result. It does not require any expertise.

In the kit, there is an incubator that incubates two dipslides simultaneously. 

 


Using a bacteria and fungi kit

Step one

Take out the slide

Cautiously remove the dipslide from the tube.

Note: On one side of the dipslide is nutrient agar, while on the other is malt Extract. Touching either of the two surfaces may cause contamination.

Step two

Collecting the sample

This depends on the method of testing that you want to use.

I. Swab testing – using the swab, apply the sample to the nutrient agar.

II. Liquid testing – submerge the dipslide into the liquid for about 10 seconds. Remove it and wait for a little for it to drain.

III. Surface testing – this is done by pressing the dipslide on top of the surface. Extra pressure may be applied by placing a finger on the bottom of the slide.

Step three

Incubating the slides

Incubate the slides for the time indicated on the kit.

Before placing the slides into the incubator for results, it is essential to ensure that all the samples are clearly labelled using stickers. This will ensure that the results are read correctly.

 

 

 

Step four

Read the results

Reading the results is easy and does not require a skill. Compare the results with the chart provided to come up with conclusions.

Microbial lab testing

Microbial lab testing involves using chemical, biological or biochemical methods to identify harmful microorganisms.

This helps prevent the diseases caused by bacterial living in the working place or at home. This makes it a significant test for all, especially those with a higher risk of infection.

If the bacteria contaminate food, they are known to cause food poisoning.

This is, therefore, a critical test that is mandatory in food processing.

Microbial food testing formats

Some of the widely used test formats include;

• Agar plates

• Real-time Polymerase Chain Reaction (PCR) tests

• Enzyme-linked immunoassay (ELISA)

• Nutrients plate

One of the biggest challenges faced while carrying out these tests is that the microorganisms are not evenly distributed in the whole sample.

While some may settle at the top of the samples, others prefer the mid, while others are concentrated in specific ingredients.

For this reason, a range of results must be obtained to get the actual result.

 


Legionella laboratory testing

Importance of the test

Legionella is a harmful bacterial that is known to cause flu-like symptoms (Pontiac fever) or pneumonia. This makes the test very important, especially with the ongoing pandemic.

Covid 19Symptoms to look out for

Some of the common symptoms to look out for, among others include;

• Mild headache

• Fever with chills

• Dry or productive cough

• Shortness of breath

• Nausea

Who is at risk?

Any person at any age is at risk of being infected. Nevertheless, people over 50 years of age are at a higher risk, predominantly those who smoke.

Other people who stand higher risk have a compromised immune system due to underlying conditions such as kidney disease, HIV/AIDS, and lung cancer.

Generally, more men are affected than women.

Collecting the sample

Samples are collected depending on the type of test to be carried out. For culture testing, sputum is collected, while for antigen testing, urine is collected.

Note: it is essential to note that various tests can be carried out to detect the same. However, the direct fluorescent antibody (DFA) test is currently not acclaimed. The blood test is no longer being used even though it is still available in some laboratories.

Antimicrobial testing

In simple terms, this is a comprehensive test used to concludethe precise antibiotics an exact fungus or bacteria is sensitive to.

After the test, the lowest concentration of drugs can inhibit the growth of the fungi or the bacteria. This is referred to as Minimal Inhibitory Concentration (MIC)

The test, therefore, makes it easy for the doctor to conclude which type of drug and the dosage that they are going to use. It is commonly used in infections that are difficult to treat.

Antimicrobial testing methods

• Automated instrument systems

• Broth dilution tests

• Antimicrobial gradient method

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